**r Fast and elegant way to calculate fold change between**

calculate fold change among samples as my experiments has 3 samples and I want to see up and down regulated genes among sample 1 vs sample 2 ,* *sample 1 vs sample 3.... Fold change is a measure describing how much a quantity changes going from an initial to a final value. For example, an initial value of 30 and a final value of 60 corresponds to a fold change of

**Fold change significance PCR RT-PCR and Real-Time PCR**

western blot data analysis Can anyone guide me on how to normalize the western blot band intensity and calculate the changes in expression of protein of interest? Below is the formula I used to calculate but m not sure whether m in the right track.... My main concern is how to calculate the fold change when I have biological replicate , I posted in biology group they said it is better I post it here How then can one calculate p-values for fold change if it is based on average

**ddCt method for qRT{PCR data analysis Bioconductor**

The functions look correct, but calculate a few by hand and ensure they match. One thing I should note is that the subtraction of the Ct values usually happens before an average is made, since generally both are in the same well. how to delete email address from facebook Divide the new amount of an item by the original amount to determine the fold change for an increase. For instance, if you have 2 armadillos in a hutch and after breeding, you have 8 armadillos, the calculation is 8/2 = 4.

**Fold change significance PCR RT-PCR and Real-Time PCR**

Fold Enrichment Method This normalization method is also called 'signal over background' or 'relative to the no-antibody control'. With this method, the ChIP signals are divided by the no-antibody signals, representing the ChIP signal as the fold increase in signal relative to the background signal. how to change address with the lott The functions look correct, but calculate a few by hand and ensure they match. One thing I should note is that the subtraction of the Ct values usually happens before an average is made, since generally both are in the same well.

## How long can it take?

### Computing Fold-change for RNAseq Data

- Fold Change Workbench - Columbia University
- change Seton Hall University Pirate Server
- Fold Change Workbench - Columbia University
- Testing significance relative to a fold-change threshold

## How To Calculate Fold Change

Divide the new amount of an item by the original amount to determine the fold change for an increase. For instance, if you have 2 armadillos in a hutch and after breeding, you have 8 armadillos, the calculation is 8/2 = 4.

- This is a great question and I've been searching for the answer myself. Here is what I've come up with: 1) take the log of the fold changes (on the 0 to infinity scale); 2) average the log values; 3) calculate the anti-log; 4) then transform to +/- values if necessary
- Yes, the correct usage is that 100% increase is the same as a two-fold increase. The reason is that when using percentages we are referring to the difference between the final amount and the initial amount as a fraction (or percent) of the original amount.
- Hmm. I am a bit confused here. So, basically, for this particular gene, the info about possible SNP is already there. My task now is to check the SNP for each sample and then try to make some correlation between gene expression and thos SNP.
- Subtraction Method 1: Calculate Change In the subtraction method, you first calculate the amount of change between the initial quantity and the final amount. Subtract the initial total from the final total to find the change .